wisp1 antibody  (R&D Systems)

Journal: Clinical Science (London, England : 1979)

Article Title: WNT1-inducible signaling pathway protein 1 regulates kidney inflammation through the NF-κB pathway

doi: 10.1042/CS20210663

Figure Lengend Snippet: ( A ) F4/80 positive cells were accumulated in mice kidney (kidney cortex) with WISP1 administration and reduced by PTL, which was verified by counting the percentage of F4/80 positive cells. ( B ) The expression of inflammation markers was up-regulated by WISP1 and alleviated by PTL treatment. ( C ) M1 and M2 marker expression. Results are expressed as the mean ± SEM relative gene expression, *** P <0.001,** P <0.01, * P <0.05. n =3–5.

Article Snippet: Recombinant human WISP1 protein, IgG control and mouse WISP1 antibody were purchased commercially (R&D Systems, Minneapolis, MN) and used at specified concentrations.

Techniques: Expressing, Marker, Gene Expression

Journal: Clinical Science (London, England : 1979)

Article Title: WNT1-inducible signaling pathway protein 1 regulates kidney inflammation through the NF-κB pathway

doi: 10.1042/CS20210663

Figure Lengend Snippet: At 48 h after administration of WISP1 and/or PTL to healthy mice, there were: ( A ) no morphology changes (Masson staining) observed based on in the kidney at 48 h after administration of WISP1 and/or PTL in healthy mice. ( B ) No collagen (PSR staining) alteration was found in the WISP1-treated mice. ( C ) Q-PCR data showed no significant changes in collagen1a1 and fibronectin. ( D ) α-SMA expression were up-regulated with WISP1 treatment, reduced by PTL. Results are expressed as the mean ± SEM relative gene expression, ** P <0.01, * P <0.05. n =3–5.

Article Snippet: Recombinant human WISP1 protein, IgG control and mouse WISP1 antibody were purchased commercially (R&D Systems, Minneapolis, MN) and used at specified concentrations.

Techniques: Staining, Expressing, Gene Expression

Journal: Clinical Science (London, England : 1979)

Article Title: WNT1-inducible signaling pathway protein 1 regulates kidney inflammation through the NF-κB pathway

doi: 10.1042/CS20210663

Figure Lengend Snippet: ( A ) The translocation of P-p65 into nucleus induced by WISP1 was inhibited by treatment of NFκB inhibitor PTL, with the inhibition on the up-regulated expression of TNF-α. ( B , C ) PTL inhibited the LPS-induced nucleus translocation of P-p65 and the up-regulated expression of inflammation markers. ( D ) Results are expressed as the mean ± SEM relative gene expression, *** P <0.001,** P <0.01. n =3–5.

Article Snippet: Recombinant human WISP1 protein, IgG control and mouse WISP1 antibody were purchased commercially (R&D Systems, Minneapolis, MN) and used at specified concentrations.

Techniques: Translocation Assay, Inhibition, Expressing, Gene Expression

Journal: Clinical Science (London, England : 1979)

Article Title: WNT1-inducible signaling pathway protein 1 regulates kidney inflammation through the NF-κB pathway

doi: 10.1042/CS20210663

Figure Lengend Snippet: ( A ) Knockdown (KD) of WISP1 by shRNA. ( B ) Knockdown of WISP1 in LPS-stimulated RAW macrophages inhibited ( B ) the translocation of P-p65 into nucleus, and up-regulated ( C ) expression of inflammation markers. *** P <0.001,** P <0.01. n =3–5.

Article Snippet: Recombinant human WISP1 protein, IgG control and mouse WISP1 antibody were purchased commercially (R&D Systems, Minneapolis, MN) and used at specified concentrations.

Techniques: Knockdown, shRNA, Translocation Assay, Expressing

Journal: Clinical Science (London, England : 1979)

Article Title: WNT1-inducible signaling pathway protein 1 regulates kidney inflammation through the NF-κB pathway

doi: 10.1042/CS20210663

Figure Lengend Snippet: ( A ) The addition of WISP1 antibody (Ab) inhibited the translocation of P-p65 induced by LPS and the expression of inflammation markers ( B,C ). ELISA showed that the up-regulation TNF-α was reduced by WISP1 knockdown and the addition of antibody. Results are expressed as the mean ± SEM relative gene expression, *** P <0.001,** P <0.01, * P <0.05. n =3–5.

Article Snippet: Recombinant human WISP1 protein, IgG control and mouse WISP1 antibody were purchased commercially (R&D Systems, Minneapolis, MN) and used at specified concentrations.

Techniques: Translocation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Knockdown, Gene Expression

Journal: Clinical Science (London, England : 1979)

Article Title: WNT1-inducible signaling pathway protein 1 regulates kidney inflammation through the NF-κB pathway

doi: 10.1042/CS20210663

Figure Lengend Snippet: The representative cell images were taken using 4× light microscope. ( A ) PTL inhibited kidney fibroblast cells proliferation induced by recombinant WISP1 protein (1 μg/ml), validated by counting cell numbers. ( B ) Wisp1 knocked-down using shRNA reduced the proliferation of kidney fibroblast cells by TNF-α, confirmed by counting cell number. ( C ) The addition of WISP1 antibody reduced the fibroblast cell growth in the presence of TNF-α, as confirmed by counting cell number. n =3.

Article Snippet: Recombinant human WISP1 protein, IgG control and mouse WISP1 antibody were purchased commercially (R&D Systems, Minneapolis, MN) and used at specified concentrations.

Techniques: Light Microscopy, Recombinant, shRNA

Journal: Clinical Science (London, England : 1979)

Article Title: WNT1-inducible signaling pathway protein 1 regulates kidney inflammation through the NF-κB pathway

doi: 10.1042/CS20210663

Figure Lengend Snippet: ( A ) STZ-induced DN mice at 18 weeks demonstrated the increased expression of TNF-α, MCP-1, CD68, α-SMA and WISP1 gene by qPCR, compared with control mice ( n =12–15 mice/group). Immunofluorescence staining indicated the elevated expression of WISP1 in DN model. Macrophage co-localized with WISP1 staining (white arrow). ( B ) High glucose-treated RAW cells expressed more WISP1, TNF-α and MCP-1 than low glucose-treated cells. ( C ) The kidneys of mice with 7 days post-UUO surgery showed an increased expression of inflammation genes, TNF-α, MCP-1 and IL6 and an up-regulation of WISP1, compared with control kidneys ( n =5 mice/group). Immunofluorescence staining indicated the elevated expression of WISP1 in UUO model. Macrophage co-localized with WISP1 staining (white arrow). *** P <0.001,** P <0.01.

Article Snippet: Recombinant human WISP1 protein, IgG control and mouse WISP1 antibody were purchased commercially (R&D Systems, Minneapolis, MN) and used at specified concentrations.

Techniques: Expressing, Control, Immunofluorescence, Staining

Journal: Clinical Science (London, England : 1979)

Article Title: WNT1-inducible signaling pathway protein 1 regulates kidney inflammation through the NF-κB pathway

doi: 10.1042/CS20210663

Figure Lengend Snippet: ( A ) Q-PCR analysis demonstrated that WISP1 antibody reduced inflammation gene expression in kidneys from UUO mice. ( B ) UUO kidneys without treatment or receiving IgG show an up-regulated F4/80 positive cells accumulation at 7 days. Administration of WISP1 antibody in UUO mice resulted in a reduction, verified by the percentage of F4/80 positive cells. ( C ) WISP1 antibody treatment rescued the degradation of IκBα expression in UUO kidneys, by Western blotting. Results are expressed as the mean ± SEM relative gene expression, *** P <0.001, ** P <0.01, * P <0.05. n =3–7.

Article Snippet: Recombinant human WISP1 protein, IgG control and mouse WISP1 antibody were purchased commercially (R&D Systems, Minneapolis, MN) and used at specified concentrations.

Techniques: Gene Expression, Expressing, Western Blot

Journal: Nature

Article Title: Metastatic niche labelling reveals tissue parenchyma stem cell features

doi: 10.1038/s41586-019-1487-6

Figure Lengend Snippet: a, Schematic of metastatic progression using labelling-4T1 cells6. b, Experimental design for RNA-seq6. c, Principle Component Analysis (PCA) diagram of CD45-Ter119- cell signatures from metastatic lungs at early (n=3, 10 mice each) and late (n=3, 5 mice each) time points. d, Venn diagram of differentially expressed genes in Cherry-niche from RNA-seq and selected factors common at early and late stages. e, Anti-Wisp1 blocking antibody treatment in vivo (n=10, from two independent experiments; data shown on a Tukey plot: box from the 25th to 75th percentiles, the bar is the median and the whiskers from smallest to largest value). f, GSEA correlation from RNA-seq data comparing early (n=3) or late (n=3) Cherry+ samples vs their respective Cherry-controls. g, Representative IF image of lung tissue (n=3 mice): mCherry-labelled micro-metastasis (red), Surfactant protein C (SP-C) (white) and DAPI (blue). Scale bars: main 100μm, inset 10μm (white arrows: mCherry labelled SP-C+ cells). h, Epcam+ cell frequency on Lin-(CD45-CD31-Ter119-) cells in distal lung (Ch-) and Cherry-niche (Ch+) estimated by FACS (n=13). i, Representative FACS plots from (h). Statistical analysis by unpaired two-tailed t-test with Welch’s correction (e), weighted Kolmogorov–Smirnov-like statistic with Benjamini-Hochberg correction (f) and paired two-tailed t-test (h). See Source Data.

Article Snippet: In selected experiments, wells were supplemented with 4-Hydroxy-TEMPO (200μM, Merck Sigma-Aldrich, Germany) or mouse anti-Wisp1 (250ng/ml, MAB1680, R&D, USA).

Techniques: RNA Sequencing Assay, Blocking Assay, In Vivo, Two Tailed Test